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Ultrafast excited state dynamics of biliverdin dimethyl Ester coordinate with zinc ions
jqchen
State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, China
Abstract:
As one of the biological endogenous pigments, biliverdin (BV) and its dimethyl ester (BVE) have extremely weak fluorescence in solution with quantum yield (QY) less than 0.01%. However, the situation reversed with the addition of zinc ions. The strength for fluorescence of BVE-Zn2+ complex is greatly enhanced and fluorescence QY can increase to ~5%. Herein, we studied ultrafast excited state dynamics of BVE-Zn2+ complex in ethanol, n-propanol, and DMSO solutions in order to reveal the mechanism of fluorescence QY enhancement. The results show that BVE can form a stable coordination complex with Zinc with 1:1 stoichiometry in solution. BVE is structurally and energetically more stable in the complex. Using picosecond time-resolve fluorescence and femtosecond transient absorption spectroscopy, we show that smaller non-radiative rate constant of BVE-Zn2+ complex in DMSO is the key to increasing its fluorescence QY and the excited state decay mechanism is also revealed. These results provide valuable information about the fluorescence property change after BVE binding to metal ions and may provide a guidance for the study of phytochromea or other fluorescence proteins in which BV/BVE act as chromophore.
Key words:  biliverdin, zinc, fluorescence, quantum yield, femtosecond transient absorption, excited state dynamics.
FundProject:
Ultrafast excited state dynamics of biliverdin dimethyl Ester coordinate with zinc ions
jqchen
State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, China
摘要:
As one of the biological endogenous pigments, biliverdin (BV) and its dimethyl ester (BVE) have extremely weak fluorescence in solution with quantum yield (QY) less than 0.01%. However, the situation reversed with the addition of zinc ions. The strength for fluorescence of BVE-Zn2+ complex is greatly enhanced and fluorescence QY can increase to ~5%. Herein, we studied ultrafast excited state dynamics of BVE-Zn2+ complex in ethanol, n-propanol, and DMSO solutions in order to reveal the mechanism of fluorescence QY enhancement. The results show that BVE can form a stable coordination complex with Zinc with 1:1 stoichiometry in solution. BVE is structurally and energetically more stable in the complex. Using picosecond time-resolve fluorescence and femtosecond transient absorption spectroscopy, we show that smaller non-radiative rate constant of BVE-Zn2+ complex in DMSO is the key to increasing its fluorescence QY and the excited state decay mechanism is also revealed. These results provide valuable information about the fluorescence property change after BVE binding to metal ions and may provide a guidance for the study of phytochromea or other fluorescence proteins in which BV/BVE act as chromophore.
关键词:  biliverdin, zinc, fluorescence, quantum yield, femtosecond transient absorption, excited state dynamics.
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