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PMOXA/PAA brushes toward on-line preconcentration for BSA in capillary electrophoresis
Yanmei Wang
Author NameAffiliationE-mail
Yanmei Wang Department of Polymer Science and Engineering, University of Science and Technology of China wangyanm@ustc.edu.cn 
Abstract:
In this work, a mixed polymer brushes coated capillary with switchable protein adsorption/desorption properties was developed and applied for on-line preconcentration of proteins. Firstly, amine-terminated poly(2-methyl-2-oxazoline) (PMOXA-NH2) and thiol-terminated poly(acrylic acid) (PAA-SH) were synthesized by cationic ring-opening polymerization (CROP) and reversible addition fragmentation chain transfer (RAFT) polymerization, respectively. Then, mixed brushes based on poly(2-methyl-2-oxazoline) (PMOXA) and poly(acrylic acid) (PAA) coated capillary were prepared by sequentially grafting of PMOXA-NH2 and PAA-SH onto fused-silica capillary inner surface through poly(dopamine) (PDA) as an anchor. The obtained PMOXA/PAA mixed brushes coated capillary or capillary raw material were characterized in terms of the thickness, surface chemical composition by using scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS). The switchable protein adsorption/desorption performance of the mixed polymer coated capillary was investigated by using fluorescence microscope under different pH and ionic strength (I). The results showed that bovine serum albumin (BSA) could be adsorbed on PMOXA/PAA mixed brushes coated capillary at pH 5.0 (I=10-5 M), and then the adsorbed BSA could be released at pH 9.0 (I=10-1 M). This switchable protein adsorption/desorption property of coated capillary were then used to preconcentrate proteins on line for increasing the detection sensitivity of BSA in capillary electrophoresis (CE). With this procedure, a sensitivity enhancement factor (SEF) more than 5000 for BSA was obtained.
Key words:  Mixed brush coating  Switchable properties  On-line preconcentration  Capillary electrophoresis  Detection sensitivity
FundProject:
PMOXA/PAA brushes toward on-line preconcentration for BSA in capillary electrophoresis
Yanmei
摘要:
In this work, a mixed polymer brushes coated capillary with switchable protein adsorption/desorption properties was developed and applied for on-line preconcentration of proteins. Firstly, amine-terminated poly(2-methyl-2-oxazoline) (PMOXA-NH2) and thiol-terminated poly(acrylic acid) (PAA-SH) were synthesized by cationic ring-opening polymerization (CROP) and reversible addition fragmentation chain transfer (RAFT) polymerization, respectively. Then, mixed brushes based on poly(2-methyl-2-oxazoline) (PMOXA) and poly(acrylic acid) (PAA) coated capillary were prepared by sequentially grafting of PMOXA-NH2 and PAA-SH onto fused-silica capillary inner surface through poly(dopamine) (PDA) as an anchor. The obtained PMOXA/PAA mixed brushes coated capillary or capillary raw material were characterized in terms of the thickness, surface chemical composition by using scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS). The switchable protein adsorption/desorption performance of the mixed polymer coated capillary was investigated by using fluorescence microscope under different pH and ionic strength (I). The results showed that bovine serum albumin (BSA) could be adsorbed on PMOXA/PAA mixed brushes coated capillary at pH 5.0 (I=10-5 M), and then the adsorbed BSA could be released at pH 9.0 (I=10-1 M). This switchable protein adsorption/desorption property of coated capillary were then used to preconcentrate proteins on line for increasing the detection sensitivity of BSA in capillary electrophoresis (CE). With this procedure, a sensitivity enhancement factor (SEF) more than 5000 for BSA was obtained.
关键词:  Mixed brush coating  Switchable properties  On-line preconcentration  Capillary electrophoresis  Detection sensitivity
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