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Effect of short telomeric DNA d[TTAGGG] on structure of single-stranded telomeric DNA d[AGGG(TTAGGG)6]
叶晓东
Author NameAffiliationE-mail
叶晓东 中国科学技术大学化学物理系 xdye@ustc.edu.cn 
Abstract:
The structures of human telomeric DNA have received much attention due to its significant biological importance. Most studies have focused on G-quadruplex structure formed by short telomeric DNA sequence, little is known about the structures of longer single-stranded telomeric DNAs. Here, we investigated the structure of DNA with a long sequence of d[AGGG(TTAGGG)6] (G6-DNA) and the effect of short telomeric DNA d[TTAGGG] (G01-DNA) on the structure of G6-DNA using sedimentation velocity technique, polyacrylamide gel electrophoresis, circular dichroism spectroscopy and UV melting experiments. The results suggest that the G6-DNA can form dimers in aqueous solutions and G01-DNA can form additional G-quadruplex structures by binding to G6-DNA. However, G01-DNA has no effect on the structure of DNA with a sequence of d[AGGG(TTAGGG)3] (G3-DNA). Our study provides new insights to the structure polymorphism of long human single-stranded telomeric DNA.
Key words:  Circular dichroism spectroscopy  G-quadruplex  Sedimentation velocity experiments
FundProject:
Effect of short telomeric DNA d[TTAGGG] on structure of single-stranded telomeric DNA d[AGGG(TTAGGG)6]
叶晓东
摘要:
The structures of human telomeric DNA have received much attention due to its significant biological importance. Most studies have focused on G-quadruplex structure formed by short telomeric DNA sequence, little is known about the structures of longer single-stranded telomeric DNAs. Here, we investigated the structure of DNA with a long sequence of d[AGGG(TTAGGG)6] (G6-DNA) and the effect of short telomeric DNA d[TTAGGG] (G01-DNA) on the structure of G6-DNA using sedimentation velocity technique, polyacrylamide gel electrophoresis, circular dichroism spectroscopy and UV melting experiments. The results suggest that the G6-DNA can form dimers in aqueous solutions and G01-DNA can form additional G-quadruplex structures by binding to G6-DNA. However, G01-DNA has no effect on the structure of DNA with a sequence of d[AGGG(TTAGGG)3] (G3-DNA). Our study provides new insights to the structure polymorphism of long human single-stranded telomeric DNA.
关键词:  Circular dichroism spectroscopy  G-quadruplex  Sedimentation velocity experiments
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