引用本文:
【打印本页】   【HTML】   【下载PDF全文】   View/Add Comment  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 3729次   下载 162 本文二维码信息
码上扫一扫!
分享到: 微信 更多
Synthesis and Adsorption Study of BSA Surface Imprinted Polymer on CdS Quantum Dots
Ping-ping Tang,Ji-bao Cai,Qing-de Su*
Author NameAffiliationE-mail
Ping-ping Tang Department of Chemistry, University of Science and Technology of China, Hefei 230026, China  
Ji-bao Cai Department of Chemistry, University of Science and Technology of China, Hefei 230026, China  
Qing-de Su* Department of Chemistry, University of Science and Technology of China, Hefei 230026, China qdsu@ustc.edu.cn 
Abstract:
A new bovine serum albumin (BSA) surface imprinting method was developed by the in-corporation of quantum dots (QDs) into molecularly imprinted polymers (MIP), which can offer shape selectivity. Preparation and adsorption conditions were optimized. Physical ap-pearance of the QDs and QDs-MIP particles was illustrated by scanning electron microscope images. Photoluminescence emission of CdS was quenched when rebinding of the template.The quenching of photoluminescence emissions is presumably due to the fluorescence reso-nance energy transfer between quantum dots and BSA template molecules. The adsorption is compiled with Langmuir isotherm, and chemical adsorption is the rate-controlling step.The maximum adsorption capacity could reach 226.0 mg/g, which is 142.4 mg/g larger than that of undoped BSA MIP. This study demonstrates the validity of QDs coupled with MIP technology for analyzing BSA.
Key words:  Quantum dots, Molecularly imprinted polymer, Bovine serum albumin, Langmuir isotherm
FundProject:
Synthesis and Adsorption Study of BSA Surface Imprinted Polymer on CdS Quantum Dots
唐苹苹,蔡继宝,苏庆德*
摘要:
开发了CdS量子点用于牛血清白蛋白(BSA)表面压印的方法,将CdS量子点掺杂进BSA的分子压印聚合物中. 实验过程中对制备条件和吸附条件进行了优化. 量子点(QDs)和量子点分子压印聚合物(QDs-MIP)的形貌用扫描电子显微镜进行了表征. 当该QDs-MIP重新结合模板分子BSA时,CdS量子点的荧光被淬灭. 荧光淬灭的原因可能是量子点与模板蛋白质分子之间的荧光共振能量转移. 该聚合物对压印分子的吸附为单分子层吸附,符合Langmuir等温吸附模型. 化学吸附为速率控制步骤. 该新型聚合物的最大吸附容量可达226.0 mg/g,比未掺杂量子点的BSA压印聚合物提高142.4 mg/g.
关键词:  量子点, 分子压印聚合物, 牛血清白蛋白, 表面压印,Langmuir等温吸附
DOI:10.1088/1674-0068/23/02/195-200
分类号: