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γ-Ray-Radiation-Scissioned Chitosan as a Gene Carrier and Its Improved in uitro Gene Transfection Performance
Fu-xing Lin,Kun Zeng,Wen-xiu Yang,Mo-zhen Wang*,Jie-lin Rong,Juan Xie,Yu Zhao*,Xue-wu Ge
Author NameAffiliationE-mail
Fu-xing Lin CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China  
Kun Zeng CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China  
Wen-xiu Yang CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China  
Mo-zhen Wang* CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China pstwmz@ustc.edu.cn 
Jie-lin Rong Department of Plastic Surgery, First A liated Hospital of Anhui Medical University, Hefei 230032, China  
Juan Xie Department of Plastic Surgery, First A liated Hospital of Anhui Medical University, Hefei 230032, China  
Yu Zhao* Department of Plastic Surgery, First A liated Hospital of Anhui Medical University, Hefei 230032, China zhaoyuzj@aliyun.com 
Xue-wu Ge CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China  
Abstract:
Chitosan (CS) is expected to be an ideal gene carrier for its high biosafety. In this work, CS with low molecular weight were prepared through the γ-ray radiation on the acetic acid solution of CS. The CS chains were scissioned under the γ-ray radiation, and the molecular weight (MW) of CS decreased with the absorbed dose. When the absorbed dose was above 30 kGy, the molecular weight of CS decreased about an order of magnitude. The γ-ray-radiation-scissioned CS can e ectively bind with plasmid (pEGFP) through complex coacervation method, forming pEGFP/ γ-ray-radiation-scissioned CS complex particles with a size of 200-300 nm. The complex particles have good stability and little cytotoxicity. The in uitro gene transfection efficiencies of the pEGFP/ γ-ray-radiation-scissioned CS complex particles were investigated by fluorescence microscope and flow cytometry. The results showed that the gene vectors using γ-ray-radiation-scissioned CS as the carrier will possess better gene transfection efficiency than those using natural high-MW CS as the carrier. The higher the absorbed dose, the smaller the MW of CS and the better transfection efficiency of the corresponding gene vector. This work provides a green and simple method on the preparation of CS-based gene vectors with high efficiency and biosafety.
Key words:  Chitosan  Biocompatibility  Radiation scission  Gene transfection
FundProject:This work was supported by the National Natural Science Foundation of China (No.81171829, No.51473152, and No.51573175) and the Fundamental Research Funds for the Central Universities (WK2060200012, WK3450000001). We also thank Prof. Li-hua Yang and Prof. Ye-zi You at the University of Science and Technology of China (USTC) for their kind help in providing experimental reagents and instruments
壳聚糖辐射裂解制备基因载体及其体外转染性能研究
林福星,曾琨,杨文秀,汪谟贞*,荣洁琳,谢娟,赵宇*,葛学武
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DOI:10.1063/1674-0068/30/cjcp1609185
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