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In situ Detection of Amide A Bands of Proteins in Water by Raman Ratio Spectrum
Cheng-qian Tang1, Ke Lin2, Xiao-guo Zhou1,3, Shi-lin Liu1
1.Hefei National Laboratory for Physical Sciences at the Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, China;2.School of Physics and Optoelectronic Engineering, Xidian University, Xi'an 710071, China;3.Synergetic Innovation Center of Quantum Information & Quantum Physics, University of Science and Technology of China, Hefei 230026, China
Abstract:
The amide A band of protein is sensitive to the hydrogen bands of amide groups of proteins. However, it is hard to distinguish the amide A band of aqueous protein in situ directly, since it overlaps with O-H stretching vibration of water. In this work, we presented a new analytical method of Raman ratio spectrum, which can extract the amide A band of proteins in water. To obtain the Raman ratio spectrum, the Raman spectrum of aqueous protein was divided by that of pure water. A mathematical simulation was employed to examine whether Raman ratio spectrum is effective. Two kinds of protein, lysozyme and α-chymotrypsin were employed. The amide A bands of them in water were extracted from Raman ratio spectra. Additionally, the process of thermal denaturation of lysozyme was detected from Raman ratio spectrum. These results demonstrated the Raman ratio spectra could be employed to study the amide A modes of proteins in water.
Key words:  Raman ratio spectrum  Amide A band  In situ  Protein  Water
FundProject:
拉曼除谱原位测量水溶液中蛋白质的酰胺A谱带
汤城骞1, 林珂2, 周晓国1,3, 刘世林1
1.中国科学技术大学合肥微尺度物质科学国家实验室, 化学物理系, 合肥 230026;2.西安电子科技大学物理与光电工程学院, 西安 710071;3.量子信息与量子科技前沿协同创新中心, 合肥 230026
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DOI:10.1063/1674-0068/29/cjcp1511240
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