引用本文:
【打印本页】   【HTML】   【下载PDF全文】   View/Add Comment  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 253次   下载 325 本文二维码信息
码上扫一扫!
分享到: 微信 更多
In situ Detection of Amide A Bands of Proteins in Water by Raman Ratio Spectrum
Cheng-qian Tang,Ke Lin,Xiao-guo Zhou,Shi-lin Liu
Author NameAffiliationE-mail
Cheng-qian Tang Hefei National Laboratory for Physical Sciences at the Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, China  
Ke Lin School of Physics and Optoelectronic Engineering, Xidian University, Xi'an 710071, China klin@xidian.edu.cn,Klin@ustc.edu.cn 
Xiao-guo Zhou Hefei National Laboratory for Physical Sciences at the Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, China;Synergetic Innovation Center of Quantum Information & Quantum Physics, University of Science and Technology of China, Hefei 230026, China  
Shi-lin Liu Hefei National Laboratory for Physical Sciences at the Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, China slliu@ustc.edu.cn 
Abstract:
The amide A band of protein is sensitive to the hydrogen bands of amide groups of proteins. However, it is hard to distinguish the amide A band of aqueous protein in situ directly, since it overlaps with O-H stretching vibration of water. In this work, we presented a new analytical method of Raman ratio spectrum, which can extract the amide A band of proteins in water. To obtain the Raman ratio spectrum, the Raman spectrum of aqueous protein was divided by that of pure water. A mathematical simulation was employed to examine whether Raman ratio spectrum is effective. Two kinds of protein, lysozyme and α-chymotrypsin were employed. The amide A bands of them in water were extracted from Raman ratio spectra. Additionally, the process of thermal denaturation of lysozyme was detected from Raman ratio spectrum. These results demonstrated the Raman ratio spectra could be employed to study the amide A modes of proteins in water.
Key words:  Raman ratio spectrum  Amide A band  In situ  Protein  Water
FundProject:
拉曼除谱原位测量水溶液中蛋白质的酰胺A谱带
汤城骞,林珂,周晓国,刘世林
摘要:
关键词:  
DOI:10.1063/1674-0068/29/cjcp1511240
分类号: