引用本文:
【打印本页】   【HTML】   【下载PDF全文】   View/Add Comment  【EndNote】   【RefMan】   【BibTex】
过刊浏览    高级检索
本文已被:浏览 198次   下载   
分享到: 微信 更多
Mesoporous MnSiO3@Fe3O4@C Nanoparticle as pH-responsive T1-T2* Dual-modal Magnetic Resonance Imaging Contrast Agent for Tumor Diagnosis
Qianwang Chen
Author NameAffiliationE-mail
Qianwang Chen 中国科大功能纳米实验室中国科学技术大学材料科学与工程系安徽合肥 230026 cqw@ustc.edu.cn 
Abstract:
Mesoporous structured MnSiO3@Fe3O4@C nanoparticles(NPs) were prepared via a facile yet efficient strategy, with negligible cytotoxicity and minor side effort. The as-prepared MnSiO3@Fe3O4@C NPs hold great potential in serving as pH-responsive T1-T2* dual-modal magnetic resonance (MR) imaging contrast agent. The released Mn2+ shortened T1 relaxation time, meanwhile the superparamagnetic Fe3O4 enhanced T2 contrast imaging. The release rate of Mn ions reach 49.07% under the condition of pH=5.0 which is similar to tumor microenvironment and organelles. Cytotoxicity assays show that MnSiO3@Fe3O4@C NPs have minor toxicity, even at high concentrations. After intravenous injection of MnSiO3@Fe3O4@C NPs, a rapid contrast enhancement in tumors was achieved with a significant enhancement of 132% 24 h after the administration. Moreover, a significant decreasement of 53.8% was witnessed in T2 MR imaging signal. It demonstrated that MnSiO3@Fe3O4@C NPs can act as both positive and negative MR imaging contrast agents. Besides, owe to pH-responsive degradation of mesoporous MnSiO3, they can also be used as potential drug systems for cancer theranostics.
Key words:  MR imaging, MnSiO3@Fe3O4@C, dual-modal, contrast agent
FundProject:
有介孔结构的MnSiO3@Fe3O4@C纳米粒子的制备以及作为pH响应的T1-T2*双模MRI造影剂的研究应用
陈乾旺
摘要:
我们通过一个简单的、温和的方案制备了平均尺寸为120 nm,具备介孔结构的纳米粒子MnSiO3@Fe3O4@C,粒子的细胞毒性微小,可忽略不计,可以用作T1-T2*双模MRI造影剂。酸性条件下MnSiO3@Fe3O4@C释放出大量的Mn2+缩短T1弛豫时间,提高成像分辨率。超顺磁性的Fe3O4可以增强T2对比成像,检测病变组织。类似于肿瘤微环境/细胞器的酸性PBS(pH = 5.0)中Mn2+的释放率达到49.07%,约为中性条件(pH = 7.4)下的10倍。释放的Mn2+通过内吞作用被细胞摄取,经肾脏排出,细胞毒性实验表明,MnSiO3@Fe3O4@C具有低的细胞毒性,即使高浓度的200 ppm MnSiO3@Fe3O4@C对HeLa细胞的毒性也相对较小。对荷瘤小鼠静脉注射定量MnSiO3@Fe3O4@C后,可以观察到一个快速增强的对比成像,给药24 h后,T1 MRI信号显著增强,达到132%,而T2信号则明显降低至53.8%,活体MR成像证明了MnSiO3@Fe3O4@C可以同时作为阳性和阴性造影剂。此外,得益于介孔MnSiO3优秀的酸敏感性,MnSiO3@Fe3O4@C可以作为一种潜在的药物载体,实现肿瘤的诊疗一体化。
关键词:  造影剂,双模,MR成像,MnSiO3@Fe3O4@C
DOI:
分类号: