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Effects of Matrix-to-analyte Ratio and Laser Energy on Peptides Ion Signals
Liu-zhu Zhou1,2, Yuan Zhu1, Xiao-yong Guo1, Wen-wu Zhao1, Hai-yang Zheng1, Xue-jun Gu1, Li Fang*1, Wei-jun Zhang1
1.Lab of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China;2.Qufu Normal University, Qufu 273165, China
Abstract:
A method of aerosol introduction for matrix-assisted laser desorption/ionization (MALDI) is described. The aerosol particles containing matrix and analyte enter directly into the aerosol time-of-flight mass spectrometer (ATOFMS) at atmospheric pressure. The scattered light signals from the aerosol particles are collected by a photomultiplier tube (PMT) and are passed on to an external electronic timing circuit, which determines particle size and is used to trigger a 266 nm pulsed Nd:YAG laser. The aerosol MALDI mass spectra and aerodynamic diameter of single particles can be obtained in real-time. Compared with other methods of liquid sample introduction, this method realizes detection of single particles and, more importantly, the sample consumption is lower. The effects of matrix-to-analyte ratio and laser pulse energy on analyte ion yield are examined. The optimal matrix-to-analyte ratio and laser energy are 50-110:1 and 200-400 μJ respectively.
Key words:  ATOFMS, MALDI, Matrix, Peptides
FundProject:
基质与分析物的摩尔比和激光能量对分析物离子信号的影
周留柱1,2, 朱元1, 郭晓勇1, 赵文武1, 郑海洋1, 顾学军1, 方黎*1, 张为俊1
1.中国科学院安徽光学精密机械研究所环境光谱学实验室,合肥,230031;2.曲阜师范大学,曲阜,273165
摘要:
介绍了一种基质辅助激光解吸电离的进样方法{气溶胶进样方法.包含基质和分析物的气溶胶粒子在大气压力下直接进入气溶胶飞行时间质谱仪,粒子产生的散射光信号由光电倍增管收集,通过外部的时序电路,给出气溶胶粒子粒径的信息并触发266 nm Nd:YAG激光器,可以同时获得气溶胶粒子的粒径和基质辅助激光解吸电离质谱.与其他的液体进样方式相比,它可以实现单粒子进样,因而样品消耗较少.主要研究了基质与分析物的摩尔比和激光能量对分析物离子信号的影响,合适的摩尔比和激光能量分别为50?110:1 和200?400 1J.
关键词:  气溶胶飞行时间质谱仪, 基质辅助激光解吸电离, 基质, 多肽
DOI:10.1360/cjcp2006.19(3).207.4
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