引用本文:
【打印本页】   【HTML】   【下载PDF全文】   View/Add Comment  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 1105次   下载 909 本文二维码信息
码上扫一扫!
分享到: 微信 更多
Effects of Matrix-to-analyte Ratio and Laser Energy on Peptides Ion Signals
Liu-zhu Zhou,Yuan Zhu,Xiao-yong Guo,Wen-wu Zhao,Hai-yang Zheng,Xue-jun Gu,Li Fang* ,Wei-jun Zhang
Author NameAffiliationE-mail
Liu-zhu Zhou Lab of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China; Qufu Normal University, Qufu 273165, China  
Yuan Zhu Lab of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China;  
Xiao-yong Guo Lab of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China;  
Wen-wu Zhao Lab of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China;  
Hai-yang Zheng Lab of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China;  
Xue-jun Gu Lab of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China;  
Li Fang* Lab of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China; fangli@aiofm.ac.cn,Tel:0551-5591152 
Wei-jun Zhang Lab of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China;  
Abstract:
A method of aerosol introduction for matrix-assisted laser desorption/ionization (MALDI) is described. The aerosol particles containing matrix and analyte enter directly into the aerosol time-of-flight mass spectrometer (ATOFMS) at atmospheric pressure. The scattered light signals from the aerosol particles are collected by a photomultiplier tube (PMT) and are passed on to an external electronic timing circuit, which determines particle size and is used to trigger a 266 nm pulsed Nd:YAG laser. The aerosol MALDI mass spectra and aerodynamic diameter of single particles can be obtained in real-time. Compared with other methods of liquid sample introduction, this method realizes detection of single particles and, more importantly, the sample consumption is lower. The effects of matrix-to-analyte ratio and laser pulse energy on analyte ion yield are examined. The optimal matrix-to-analyte ratio and laser energy are 50-110:1 and 200-400 μJ respectively.
Key words:  ATOFMS, MALDI, Matrix, Peptides
FundProject:
基质与分析物的摩尔比和激光能量对分析物离子信号的影
周留柱,朱元,郭晓勇,赵文武,郑海洋,顾学军,方黎*,张为俊
摘要:
介绍了一种基质辅助激光解吸电离的进样方法{气溶胶进样方法.包含基质和分析物的气溶胶粒子在大气压力下直接进入气溶胶飞行时间质谱仪,粒子产生的散射光信号由光电倍增管收集,通过外部的时序电路,给出气溶胶粒子粒径的信息并触发266 nm Nd:YAG激光器,可以同时获得气溶胶粒子的粒径和基质辅助激光解吸电离质谱.与其他的液体进样方式相比,它可以实现单粒子进样,因而样品消耗较少.主要研究了基质与分析物的摩尔比和激光能量对分析物离子信号的影响,合适的摩尔比和激光能量分别为50?110:1 和200?400 1J.
关键词:  气溶胶飞行时间质谱仪, 基质辅助激光解吸电离, 基质, 多肽
DOI:10.1360/cjcp2006.19(3).207.4
分类号: