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High-Sensitive Glucose Biosensor Based on Ionic Liquid Doped Polyaniline/Prussian Blue Composite Film
Yao Yao,Shou-guo Wu*,Hai-hong Xu,Li-wen Wang
Author NameAffiliationE-mail
Yao Yao Department of Chemistry, University of Science and Technology of China, Hefei 230026, China  
Shou-guo Wu* Department of Chemistry, University of Science and Technology of China, Hefei 230026, China sgwu@ustc.edu.cn 
Hai-hong Xu Clinical Laboratory, Hefei Third People's Hospital, Hefei 230022, China  
Li-wen Wang Department of Chemistry, University of Science and Technology of China, Hefei 230026, China  
Abstract:
The Prussian blue/ionic liquid-polyaniline/multiwall carbon nanotubes (PB/IL-PANI/MWNTs) composite film was fabricated by using cyclic voltammetry. The ion liquid acting as a lubricating agent, could enhance the electron delocalization degree and reduce the struc-tural defects of the polyaniline. The surface morphology of the composite film revealed that the PB nanoparticles have smaller size than that in pure PB film. Due to the introduction of ion liquid, the PB/IL-PANI/MWNTs composite film showed wonderful synergistic effect which can remarkably enhance sensitivity, expand linear range and broaden acidic adapt-ability for hydrogen peroxide detection. The composite film demonstrated good stability in neutral solution contrast to pure PB film, with a linear range from 2.5 μmol/L to 0.5 mmol/L and a high sensitivity of 736.8 μA·(mmol/L)-1·cm-2 for H2O2 detection. Based on the com-posite film, an amperometric glucose biosensor was then fabricated by immobilizing glucose oxidase. Under the optimal conditions, the biosensor also exhibits excellent response to glucose with the linear range from 12.5 μmol/L to 1.75 mmol/L and a high sensitivity of 94.79 μA (mmol/L)-1·cm-2 for H2O2. The detection limit was estimated 1.1 μmol/L. The resulting biosensor was applied to detect the blood sugar in human serum samples without any pretreatment, and the results were comparatively in agreement with the clinical assay.
Key words:  Hydrogen peroxide, Prussian blue, Ionic liquid, Glucose biosensor
FundProject:
离子液体掺杂聚苯胺/普鲁士蓝复合膜的高灵敏葡萄糖传感器
姚瑶,吴守国*,徐海红,王立文
摘要:
利用经典的循环伏安法制备出普鲁士蓝/离子液体-聚苯胺/多壁碳纳米管(PB/IL-PANI/MWNTs)复合膜.离子液体为润滑剂,能够显著增强聚苯胺链的电子去极化度并减少其链的结构缺陷.离子液 体的引入使该复合膜在H2O2检测中呈现出更优异的特性,线性范围为2.5~5.0 mmol/L,灵敏度为736.8 μA·(mmol/L)-1·cm-2,复合膜比纯PB 膜在中性溶液中稳定性好;传感器在12.5~1.75 mmol/L对葡萄糖也呈现很好的线性关系,检测限为1.1 μmol/L(信噪比为3),灵敏度为94.79 μA·(mmol/L)-1·cm-2.该传感器还用于检测未经过任何预处理的人血清中的血糖含量,结果和医院提供的值吻合较好.
关键词:  过氧化氢,普鲁士蓝,离子液体,葡萄糖传感器
DOI:10.1063/1674-0068/28/cjcp1504058
分类号: