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Cascade Enzymatic Hydrolysis Coupling with Ultra ne Grinding Pretreatment for Sugarcane Bagasse Sacchari cation
Zheng-qiu Yuan,Jin-xing Long,Tie-jun Wang*,Yu-qin Li*,Qi Zhang,Long-long Ma
Author NameAffiliationE-mail
Zheng-qiu Yuan Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Science, Guangzhou 510640, ChinaUniversity of Chinese Academy of Science, Beijing 100049, China  
Jin-xing Long Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Science, Guangzhou 510640, China  
Tie-jun Wang* Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Science, Guangzhou 510640, China wangtj@ms.giec.ac.cn 
Yu-qin Li* School of Chemical Engineering, Xiangtan University, Xiangtan 411105, China yuqinli2004@xtu.edu.cn 
Qi Zhang Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Science, Guangzhou 510640, China  
Long-long Ma Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Science, Guangzhou 510640, China  
Abstract:
The biorefinery process for sugarcane bagasse saccharification generally requires significant accessibility of cellulose. We reported a novel method of cascade cellulase enzymatic hydrolysis coupling with ultrafine grinding pretreatment for sugarcane bagasse saccharification. Three enzymatic hydrolysis modes including single cellulase enzymatic hydrolysis, mixed cellulase enzymatic hydrolysis, and cascade cellulase enzymatic hydrolysis were compared. The changes on the functional group and surface morphology of bagasse during cascade cellulase enzymatic hydrolysis were also examined by FT-IR and SEM respectively. The results showed that cascade enzymatic hydrolysis was the most efficient way to enhance the sugarcane bagasse sacchari cation. More than 65% of reducing sugar yield with 90.1% of glucose selectivity was achieved at 50 oC, pH=4.8 for 72 h (1200 r/min) with cellulase I of 7.5 FPU/g substrate and cellulase II of 5 FPU/g substrate.
Key words:  Sugarcane bagasse, Ultrafine grinding pretreatment, Cascade enzymatic hydrolysis, Reducing sugars, Glucose
FundProject:
串联酶解耦合超微粉碎法对蔗渣酶解的促进作用
袁正求,龙金星,王铁军*,李玉芹*,张琦,马隆龙
摘要:
报道了一种新颖的串联酶解(先酶I后酶II)耦合超微粉碎方法,并系统研究了其对蔗渣酶水解的促进作用. 通过对三种酶解方法(单酶I、单酶II、先酶I后酶II) 的比较发现,串联酶解对蔗渣酶解最为有效. 超微粉碎能破坏蔗渣细胞结构,使纤维素充分暴露出来便于酶解.在串联酶解耦合超微粉碎模式下,蔗渣在反应温度50 oC,pH=4.8,酶I(7.5 FPU/g底物)和酶II(5.0 FPU/g底物),摇床速率1200 r/min条件下反应72 h后,酶水解能得到65%的还原糖浓度,其中葡萄糖选择性为90.1%.
关键词:  蔗渣,超微粉碎,串联酶解,还原糖
DOI:10.1063/1674-0068/28/cjcp1502014
分类号: