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Real-Time Characterization of Fibrinogen Interaction with Modified Titanium Dioxide Film by Quartz Crystal Microbalance with Dissipation
An-sha Zhao1, Zhao Wang1, Xiao-hua Zhu1, Manfred F. Maitz2, Nan Huang*1
1.Key Laboratory of Advanced Materials Technology of Ministry of Education, Department of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China;2.Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Dresden, Germany
Abstract:
The adsorption of fibrinogen can be used as a quick indicator of surface haemocompatibility because of its prominent role in coagulation and platelet adhesion. In this work the molec-ular interaction between flbrinogen and a modified titanium oxide surface/platelet has been studied by quartz crystal microbalance with dissipation (QCM-D) in situ. In order to further characterize the conformation of adsorbed flbrinogen, αC and γ-chain antibody were used to check the orientation and denaturation of flbrinogen on solid surface. QCM-D investiga-tions revealed the flbrinogen have the trend to adsorb on hydrophilic surface in a side-on orientation by positively charged αC domains, which would reduce the exposure of platelet bonding site on γ chain and enable less platelet adhesion and be activated. These obser-vations suggest that certain conformations of adsorbed flbrinogen are less platelet adhesive than others, which opens a possibility for creating a non-platelet adhesive substrates.
Key words:  Quartz crystal microbalance with dissipation, Fibrinogen adsorption, Confor-mation change, Blood platelet
FundProject:
利用石英微天平技术实时监控纤维蛋白原与改性后氧化钛薄膜的界面反应
赵安莎1, 王昭1, 朱晓华1, Manfred F. Maitz2, 黄楠*1
1.西南交通大学材料先进技术教育部重点实验室,材料科学与工程学院,成都 610031;2.德累斯顿莱布尼茨高分子研究所,德国德累斯顿马克斯伯格曼生物材料中心,德累斯顿
摘要:
利用石英微天平技术实时研究了纤维蛋白原与改性后氧化钛薄膜的界面分子作用. 纤维蛋白原αC和γ链特异性抗体被用来进一步表征和检测纤维蛋白原在界面的吸附形态及构象变化. 结果显示纤维蛋白原在亲水性表面更倾向于通过αC结构域直立着吸附,这种吸附方式减少了位于纤维蛋白原γ链上血小板结合位点的暴露从而减少了血小板的粘附及激活.
关键词:  石英微天平技术,纤维蛋白原吸附,构象变化,血小板
DOI:10.1063/1674-0068/27/03/355-360
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