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Interaction Study between DNA and Histone Proteins on Single-molecule Level using Atomic Force Microscopy
Yu-ying Liu*1, Peng-ye Wang2, Shuo-xing Dou2, Hong-feng Lv1
1.College of Science, China Agricultural University, Beijing 100083, China;2.Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China
Abstract:
DNA and histone protein are important in the formation of nucleosomal arrays, which are the first packaging level of DNA into a more compact chromatin structure. To characterize the interactions of DNA and histone proteins, we reconstitute nucleosomes using lambda DNA and whole histone proteins by dialysis and perform direct atomic force microscopy (AFM) imaging. Compared with non-specific DNA and histone binding, nucleosomes are formed within the assembled “beads-on-a-string” nucleosomal array by dialysis. These observations facilitate the establishment of the molecular mechanisms of nucleosome and demonstrate the capability of AFM for protein-DNA interaction analysis.
Key words:  DNA, Histone, Atomic force microscopy, Single molecule, Dialysis
FundProject:
利用原子力显微镜研究在单分子水平上DNA与组蛋白的相互作用
刘玉颖*1, 王鹏业2, 窦硕星2, 吕洪凤1
1.中国农业大学理学院,北京100083;2.中国科学院物理研究所软物质物理实验室,北京100190
摘要:
核小体是DNA被压缩成染色质的第一级压缩结构. 为了更深刻描述DNA与组蛋白的相互作用,利用透析方法将DNA和组蛋白构建成核小体,并且应用原子力显微镜进行单分子水平上的观察. 实验结果表明利用透析方法得到了核小体的“串珠型排列”,并且对在两种不同的结合条件下DNA与组蛋白的相互作用进行了观察和比较.
关键词:  DNA,组蛋白,原子力显微镜,单分子,透析
DOI:10.1063/1674-0068/27/01/115-120
分类号: