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Methanol Perturbing Modeling Cell Membranes Investigated using Linear and Nonlinear Vibrational Spectroscopy (cited: 3)
Kangzhen Tian,Hongchun Li,Shuji Ye*
Author NameAffiliationE-mail
Kangzhen Tian Department of Physics, University of Science and Technology of China, Hefei 230026, China  
Hongchun Li Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, China  
Shuji Ye* Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, ChinaHefei National Laboratory for Physical Sciences at the Microscale, University of Science and Tech-nology of China, Hefei 230026, China shujiye@ustc.edu.cn 
Abstract:
Cell membranes play a crucial role in many biological functions of cells. A small change in the composition of cell membranes can strongly influence the functions of membrane-associated proteins, such as ion and water channels, and thus mediate the chemical and physical balance in cells. Such composition change could originate from the introduction of short-chain alcohols, or other anesthetics into membranes. In this work, we have applied sum frequency generation vibrational spectroscopy (SFG-VS), supplemented by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), to investigate interaction between methanol and 1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine (d54-DMPC) lipid bilayers. Lipid's hydrocarbon interior is deuterated while its head group is hydrogenated. At the same time, CH3 symmetric stretch from methanol and lipid head amine group has different frequency, thus we can distinguish the behaviors of methanol, lipid head amine group, and lipid hydrocarbon interior. Based on the spectral feature of the bending mode of the water molecules replaced by methanol, we determined that the methanol molecules are intercalated into the region between amine and phosphate groups at the lipid hydrophilic head. The dipole of CH3 groups of methanol and lipid head, and the water O-H all adopt the same orientation directions. The introduction of methanol into the lipid hydrophilic head group can strongly perturb the entire length of the alkyl chains, resulting that the signals of CD3 and CD3 groups from both leaflets can not cancel each other.
Key words:  Sum frequency generation vibrational spectroscopy, Cell membrane, Methanol, Interaction, Mechanism
FundProject:
线性和非线性光谱研究甲醇分子对生物膜的微扰作用 (cited: 3)
田康振,李红春,叶树集*
摘要:
利用和频光谱并结合衰减全反射傅立叶红外光谱技术研究了甲醇和1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine (d54-DMPC)磷脂双层膜之间的相互作用. 研究结果表明甲醇与生物膜作用后,嵌入到磷脂分子头部的胺基和磷酸基间的空间内,并与磷脂分子头部甲基以及与胺基附近水分子采用相似的取向方向. 嵌入磷脂分子头部的甲醇分子能扰动磷脂分子的整个疏水碳链.
关键词:  和频光谱,生物膜,甲醇,相互作用,机理
DOI:10.1063/1674-0068/26/01/27-34
分类号: