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Influence Factors on Particle Growth for On-line Aerosol Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
Wei-wei Xia*,Ru-fang Ti,Zi-liang Zhang,Hai-yang Zheng,Li Fang
Author NameAffiliationE-mail
Wei-wei Xia* Laboratory of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China xww0901@163.com 
Ru-fang Ti Laboratory of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China  
Zi-liang Zhang Laboratory of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China  
Hai-yang Zheng Laboratory of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China  
Li Fang Laboratory of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China  
Abstract:
An evaporation/condensation flow cell was developed and interfaced with the matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometer for on-line bioaerosol detection and characterization, which allows matrix addition by condensation onto the laboratory-generated bioaerosol particles. The final coated particle exiting from the con-denser is then introduced into the aerodynamic particle sizer spectrometer or home-built aerosol laser time-of-flight mass spectrometer, and its aerodynamic size directly effects on the matrix-to-analyte molar ratio, which is very important for MALDI technique. In order to observe the protonated analyte molecular ion, and then determine the classification of bi-ological aerosols, the matrix-to-analyte molar ratio must be appropriate. Four experimental parameters, including the temperature of the heated reservoir, the initial particle size, its number concentration, and the matrix material, were tested experimentally to analyze their influences on the final particle size. This technique represents an on-line system of detec-tion that has the potential to provide rapid and reliable identification of airborne biological aerosols.
Key words:  Matrix-assisted laser desorption/ionization, Particle growth, Homogeneous condensation, Evaporation/condensation flow cell
FundProject:
Influence Factors on Particle Growth for On-line Aerosol Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
夏玮玮*,提汝芳,张子良,郑海洋,方黎
摘要:
利用自行研制的蒸发/冷凝实验装置作为基质辅助激光解吸/电离飞行时间质谱仪的特种接口,通过冷凝方式在线添加基质,冷凝管出口处的最终粒子直接引入空气动力学粒径分析仪或自行研制的飞行时间质谱仪,可对实验室产生的生物气溶胶粒子进行实时检测. 同时研究了最终粒子粒径大小的决定因素:加热池温度、初始粒子大小及其粒子数浓度及基质种类,获得了探测分析物分子离子峰所需的实验条件.
关键词:  基质辅助激光解吸/电离,粒径生长,蒸发/冷凝,均质成核
DOI:10.1088/1674-0068/23/03/269-273
分类号: