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Mode of Myosin Transportation in Living Cells Studied by Single Particle Tracking
Zhang-yi Liang,Ning Xu,Ying-hua Guan,You-yi Zhang,Xin-sheng Zhao
Author NameAffiliation
Zhang-yi Liang Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China 
Ning Xu Institute of Vascular Medicine, Peking University Third Hospital, and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing 100083, China 
Ying-hua Guan Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China 
You-yi Zhang Institute of Vascular Medicine, Peking University Third Hospital, and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing 100083, China 
Xin-sheng Zhao Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China 
Abstract:
The transport of internalized ?1A-adrenergic receptor (?1A-AR) by myosin protein in live cells was studied. The technique of single particle tracking by °uorescence imaging with high temporal and spatial resolution was used. The endosomes of ?1A-AR were transported along actin ˉlaments in a step-by-step mode. The average step-size in di?erent time resolutions is consistent with the step-size of myosin assay in vitro. With the simulation of the stepwise traces in di?erent time resolutions, we found that the kinetic process of each step is in coherence with the single myosin assay in vitro.
Key words:  Single particle tracking, Live cell, ?1A-adrenergic receptor, Myosin assay
FundProject:国家自然科学基金
利用单粒子追踪技术研究活细胞内肌球蛋白的运输模式
梁璋仪,徐宁,关英华,张幼怡*,赵新生*
摘要:
利用单个粒子追踪技术研究?1A肾上腺素受体在活细胞内的转运.?1A肾上腺素受体在细胞内沿微丝骨架的转运表现为步进式模式,平均步长与体外对肌球蛋白的研究相一致.对不同时间分辨率下数据的模拟分析结果表明,肌球蛋白在细胞内运输内涵体的单步动力学与单个肌球蛋白在体外的报道相一致.
关键词:  单粒子追踪,活细胞,?1A-肾上腺素受体,肌球蛋白,步进,模拟
DOI:10.1088/1674-0068/20/04/445-448
分类号: