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单个人类端粒重复序列d(TTAGGG)对长链端粒DNA-d[AGGG(TTAGGG)6]结构影响的研究
光天磊1, 高亚婷1, 叶晓东1,2
1.中国科学技术大学化学物理系,合肥微尺度物质科学国家研究中心,合肥 230026;2.中国科学技术大学,中国科学院软物质化学重点实验室,合肥 230026
摘要:
本文使用超速离心沉降速度法、聚丙烯酰胺凝胶电泳和圆二色光谱和紫外吸收检测熔融实验研究了长序列端粒DNA-d[AGGG(TTAGGG)6](G6-DNA)的结构以及单个重复序列DNA-d(TTAGGG)(G01-DNA)对G6-DNA结构的影响. 结果表明G6-DNA可以在水溶液中形成二聚体,并且G01-DNA可以通过与G6-DNA结合形成额外的G-四链体结构,从而改变单体与二聚体的平衡. 然而,G01-DNA对序列为d[AGGG(TTAGGG)3](G3-DNA)的结构没有影响. 该研究为长序列单链端粒DNA的结构多样性提供了新的见解.
关键词:  圆二色谱,G-四链体,沉降速度实验
DOI:10.1063/1674-0068/31/cjcp1804069
分类号:
基金项目:
Effect of a Single Repeat Sequence of the Human Telomere d(TTAGGG) on Structure of Single-Stranded Telomeric DNA d[AGGG(TTAGGG)6]
Tian-lei Guang1, Ya-ting Gao1, Xiao-dong Ye*1,2
1.Hefei National Laboratory for Physical Sciences at the Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, China;2.CAS Key Laboratory of Soft Matter Chemistry, University of Science and Technology of China, Hefei 230026, China
Abstract:
The structures of human telomeric DNA have received much attention due to its significant biological importance. Most studies have focused on G-quadruplex structure formed by short telomeric DNA sequence, but little is known about the structures of long singlestranded telomeric DNAs. Here, we investigated the structure of DNA with a long sequence of d[AGGG(TTAGGG)6] (G6-DNA) and the effect of a single repeat sequence d(TTAGGG) (G01-DNA) on the structure of G6-DNA using sedimentation velocity technique, polyacrylamide gel electrophoresis, circular dichroism spectroscopy, and UV melting experiments. The results suggest that the G6-DNA can form dimers in aqueous solutions and G01-DNA can form additional G-quadruplex structures by binding to G6-DNA. However, G01-DNA has no effect on the structure of DNA with a sequence of d[AGGG(TTAGGG)3] (G3-DNA). Our study provides new insights into the structure polymorphism of long human single-stranded telomeric DNA.
Key words:  Circular dichroism spectroscopy, G-guadruplex, Sedimentation velocity experiments