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Sensitive and Label-Free Detection of Protein Secondary Structure by Surface-Enhanced Raman Spectroscopy Using Ag Nanorod Substrate
Nie xinming
作者单位E-mail
Nie xinming 江苏师范大学 nxinming@jsnu.edu.cn 
摘要:
Proteins and peptides perform a vital role in living systems, however it remains a challenge for accurate description of proteins at the molecular level. Although surface enhanced Raman spectroscopy (SERS) can provide the intrinsic fingerprint information of samples with ultrahigh sensitivity, it suffers from the reproducibility and reliability issues. Herein, we demonstrate that the silver nanorod (AgNR) array fabricated by an oblique angle deposition (OAD) method is a powerful substrate for SERS to probe the protein secondary structures without exogenous labels. With this method, the SERS signals of two typical proteins (lysozyme and cytochrome c) are successfully obtained. Besides, by analyzing the spectral signals of the amide I and amide III of protein backbone, the influence of concentration on the folding status of proteins has been elucidated: with the concentration increasing, the components of ?-helix and β-sheet structures of lysozyme increase while the secondary structures of cytochrome c almost keep constant. The SERS method in this paper offers an effective optical marker to characterize the structures of proteins.
关键词:  Silver nanorod array, Surface-enhanced Raman spectroscopy, Protein
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Sensitive and Label-Free Detection of Protein Secondary Structure by Surface-Enhanced Raman Spectroscopy Using Ag Nanorod Substrate
Xingming Nie
Abstract:
Proteins and peptides perform a vital role in living systems, however it remains a challenge for accurate description of proteins at the molecular level. Although surface enhanced Raman spectroscopy (SERS) can provide the intrinsic fingerprint information of samples with ultrahigh sensitivity, it suffers from the reproducibility and reliability issues. Herein, we demonstrate that the silver nanorod (AgNR) array fabricated by an oblique angle deposition (OAD) method is a powerful substrate for SERS to probe the protein secondary structures without exogenous labels. With this method, the SERS signals of two typical proteins (lysozyme and cytochrome c) are successfully obtained. Besides, by analyzing the spectral signals of the amide I and amide III of protein backbone, the influence of concentration on the folding status of proteins has been elucidated: with the concentration increasing, the components of ?-helix and β-sheet structures of lysozyme increase while the secondary structures of cytochrome c almost keep constant. The SERS method in this paper offers an effective optical marker to characterize the structures of proteins.
Key words:  Silver nanorod array, Surface-enhanced Raman spectroscopy, Protein