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磷酸钾缓冲溶液与模型细胞膜相互作用的和频光谱
杨未来,田康振,叶树集*
作者单位E-mail
杨未来 中国科学技术大学合肥微尺度物质科学国家实验室化学物理系量子信息和量子物理协同创新中心合肥230026  
田康振 中国科学技术大学合肥微尺度物质科学国家实验室化学物理系量子信息和量子物理协同创新中心合肥230026  
叶树集* 中国科学技术大学合肥微尺度物质科学国家实验室化学物理系量子信息和量子物理协同创新中心合肥230026 shujiye@ustc.edu.cn 
摘要:
利用和频光谱技术详细研究了磷酸钾缓冲溶液与带负电荷的生物仿生膜(d54-DMPG磷脂双层膜)相互作用的实时过程.通过监控CD2、CD3、磷脂分子头部的磷酸根以及羰基官能团的光谱信号随加入磷酸钾缓冲溶液的实时变化,获得了磷脂双层膜分子结构的动力学变化.结果表明K+能够结合到细胞膜上,并且很快地引起了CD2、CD3、磷脂头部磷酸根以及羰基官能团信号的变化.根据各官能团的和频信号响应,磷酸钾缓冲溶液很可能是通过在双层膜中形成环形气孔来与磷脂双层膜发生作用.该结果可以很好地解释磷酸钾缓冲溶液环境下的离子协助蛋白质的跨膜过程.
关键词:  和频光谱,磷酸钾缓冲溶液,磷脂双层膜,环形气孔,相互作用
DOI:10.1063/1674-0068/28/cjcp1504070
分类号:
基金项目:
Interaction between Potassium Phosphate Bu er Solution and Modeling Cell Membrane Investigated by Sum Frequency Generation Vibrational Spectroscopy (cited: 1)
Weilai Yang,Kangzhen Tian,Shuji Ye*
Abstract:
Potassium phosphate buffer solution has been widely used in the biological experiments, which represents an important process of the interaction between ions and biomolecules, yet the in fluences of potassium phosphate on biomolecules such as the cell membrane are still poorly understood at the molecular level. In this work, we have applied sum frequency generation vibrational spectroscopy and carried out a detailed study on the interaction between potassium phosphate buffer solution (PBS) and negative 1,2-dimyristoyl-d54-sn-glycero-3-[phospho-rac-(1-glycerol)] (d54-DMPG) lipid bilayer in real time. The PBS-induced dynamic change in the molecular structure of d54-DMPG lipid bilayer was monitored using the spectral features of CD2, CD3, lipid head phosphate, and carbonyl groups for the first time. It is found that K+ can bind to the cell membrane and cause the signal change of CD2, CD3, lipid head phosphate, and carbonyl groups quickly. Potassium PBS interacts with lipid bilayers most likely by formation of toroidal pores inside the bilayer matrix. This result can provide a molecular basis for the interpretation of the effect of PBS on the ion-assisted transport of protein across the membrane.
Key words:  Sum frequency generation, Potassium phosphate buffer, Lipid bilayer, Toroidal pore, Interaction