Effects of Matrix-to-analyte Ratio and Laser Energy on Peptides Ion Signals
- Received Date: 2005-04-04
- ATOFMS, MALDI, Matrix, Peptides
Abstract: A method of aerosol introduction for matrix-assisted laser desorption/ionization (MALDI) is described. The aerosol particles containing matrix and analyte enter directly into the aerosol time-of-flight mass spectrometer (ATOFMS) at atmospheric pressure. The scattered light signals from the aerosol particles are collected by a photomultiplier tube (PMT) and are passed on to an external electronic timing circuit, which determines particle size and is used to trigger a 266 nm pulsed Nd:YAG laser. The aerosol MALDI mass spectra and aerodynamic diameter of single particles can be obtained in real-time. Compared with other methods of liquid sample introduction, this method realizes detection of single particles and, more importantly, the sample consumption is lower. The effects of matrix-to-analyte ratio and laser pulse energy on analyte ion yield are examined. The optimal matrix-to-analyte ratio and laser energy are 50-110:1 and 200-400 μJ respectively.
|Citation:||Liu-zhu Zhou, Yuan Zhu, Xiao-yong Guo, Wen-wu Zhao, Hai-yang Zheng, Xue-jun Gu, Li Fang, Wei-jun Zhang. Effects of Matrix-to-analyte Ratio and Laser Energy on Peptides Ion Signals[J]. Chinese Journal of Chemical Physics , 2006, 19(3): 207-210. doi: 10.1360/cjcp2006.19(3).207.4|