Yao Yao, Shou-guo Wu, Hai-hong Xu, Li-wen Wang. High-Sensitive Glucose Biosensor Based on Ionic Liquid Doped Polyaniline/Prussian Blue Composite Film[J]. Chinese Journal of Chemical Physics , 2015, 28(6): 755-761. doi: 10.1063/1674-0068/28/cjcp1504058
Citation: Yao Yao, Shou-guo Wu, Hai-hong Xu, Li-wen Wang. High-Sensitive Glucose Biosensor Based on Ionic Liquid Doped Polyaniline/Prussian Blue Composite Film[J]. Chinese Journal of Chemical Physics , 2015, 28(6): 755-761. doi: 10.1063/1674-0068/28/cjcp1504058

High-Sensitive Glucose Biosensor Based on Ionic Liquid Doped Polyaniline/Prussian Blue Composite Film

doi: 10.1063/1674-0068/28/cjcp1504058
Funds:  This work was supported by the National Basic Re-search Program of China (No.933900).
  • Received Date: 2015-04-21
  • The Prussian blue/ionic liquid-polyaniline/multiwall carbon nanotubes (PB/IL-PANI/MWNTs) composite film was fabricated by using cyclic voltammetry. The ion liquid acting as a lubricating agent, could enhance the electron delocalization degree and reduce the struc-tural defects of the polyaniline. The surface morphology of the composite film revealed that the PB nanoparticles have smaller size than that in pure PB film. Due to the introduction of ion liquid, the PB/IL-PANI/MWNTs composite film showed wonderful synergistic effect which can remarkably enhance sensitivity, expand linear range and broaden acidic adapt-ability for hydrogen peroxide detection. The composite film demonstrated good stability in neutral solution contrast to pure PB film, with a linear range from 2.5 μmol/L to 0.5 mmol/Land a high sensitivity of 736.8 μA·(mmol/L)-1·cm-2 for H2O2 detection. Based on the com-posite film, an amperometric glucose biosensor was then fabricated by immobilizing glucose oxidase. Under the optimal conditions, the biosensor also exhibits excellent response to glucose with the linear range from 12.5 μmol/L to 1.75 mmol/L and a high sensitivity of 94.79 μA (mmol/L)-1·cm-2 for H2O2. The detection limit was estimated 1.1 μmol/L. The resulting biosensor was applied to detect the blood sugar in human serum samples without any pretreatment, and the results were comparatively in agreement with the clinical assay.
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High-Sensitive Glucose Biosensor Based on Ionic Liquid Doped Polyaniline/Prussian Blue Composite Film

doi: 10.1063/1674-0068/28/cjcp1504058
Funds:  This work was supported by the National Basic Re-search Program of China (No.933900).

Abstract: The Prussian blue/ionic liquid-polyaniline/multiwall carbon nanotubes (PB/IL-PANI/MWNTs) composite film was fabricated by using cyclic voltammetry. The ion liquid acting as a lubricating agent, could enhance the electron delocalization degree and reduce the struc-tural defects of the polyaniline. The surface morphology of the composite film revealed that the PB nanoparticles have smaller size than that in pure PB film. Due to the introduction of ion liquid, the PB/IL-PANI/MWNTs composite film showed wonderful synergistic effect which can remarkably enhance sensitivity, expand linear range and broaden acidic adapt-ability for hydrogen peroxide detection. The composite film demonstrated good stability in neutral solution contrast to pure PB film, with a linear range from 2.5 μmol/L to 0.5 mmol/Land a high sensitivity of 736.8 μA·(mmol/L)-1·cm-2 for H2O2 detection. Based on the com-posite film, an amperometric glucose biosensor was then fabricated by immobilizing glucose oxidase. Under the optimal conditions, the biosensor also exhibits excellent response to glucose with the linear range from 12.5 μmol/L to 1.75 mmol/L and a high sensitivity of 94.79 μA (mmol/L)-1·cm-2 for H2O2. The detection limit was estimated 1.1 μmol/L. The resulting biosensor was applied to detect the blood sugar in human serum samples without any pretreatment, and the results were comparatively in agreement with the clinical assay.

Yao Yao, Shou-guo Wu, Hai-hong Xu, Li-wen Wang. High-Sensitive Glucose Biosensor Based on Ionic Liquid Doped Polyaniline/Prussian Blue Composite Film[J]. Chinese Journal of Chemical Physics , 2015, 28(6): 755-761. doi: 10.1063/1674-0068/28/cjcp1504058
Citation: Yao Yao, Shou-guo Wu, Hai-hong Xu, Li-wen Wang. High-Sensitive Glucose Biosensor Based on Ionic Liquid Doped Polyaniline/Prussian Blue Composite Film[J]. Chinese Journal of Chemical Physics , 2015, 28(6): 755-761. doi: 10.1063/1674-0068/28/cjcp1504058

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