Wei Deng, Bin Zheng, Wei Ding, Hong Zhu, Hao-jun Liang. Metal-Triggered DNA Folding by Different Mechanisms[J]. Chinese Journal of Chemical Physics , 2015, 28(5): 630-638. doi: 10.1063/1674-0068/28/cjcp1503051
Citation: Wei Deng, Bin Zheng, Wei Ding, Hong Zhu, Hao-jun Liang. Metal-Triggered DNA Folding by Different Mechanisms[J]. Chinese Journal of Chemical Physics , 2015, 28(5): 630-638. doi: 10.1063/1674-0068/28/cjcp1503051

Metal-Triggered DNA Folding by Different Mechanisms

doi: 10.1063/1674-0068/28/cjcp1503051
  • Received Date: 2015-03-27
  • Metal-mediated base pairs by the interaction between metal ions and artificial bases in oligonucleotides has been widely used in DNA nanotechnology and biosensing technique. Using isothermal titration calorimetry, circular dichroism spectroscopy and fluorescence spectroscopy, the folding process of T-C-rich oligonucleotides (TCO) induced by Hg2+ and Ag+ with the synthetic sequence d(T6C6T6C6T6C6T6) was studied and analyzed. Although thermodynamic data predict that TCO should initially fold into a relatively stable hairpin through two possible pathways of conformational transitions whether Hg2+ or Ag+ were added at first, the mechanisms and final products between the two are entirely different from isothermal titration calorimetry outcomes. When Hg2+ were added first, the haipin was formed through T-Hg-T structure with further stabilization by C-Ag-C after Ag+ addition. However, it is proposed that an unusual metal-base pair for Ag+ binding is generated instead classical C-Ag-C when Ag+ was injected first. Moreover, further confirmation of this unconventional metal-base pair T-Ag-C was verified by circular dichroism and fluorescence spectroscopy.
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通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Metal-Triggered DNA Folding by Different Mechanisms

doi: 10.1063/1674-0068/28/cjcp1503051

Abstract: Metal-mediated base pairs by the interaction between metal ions and artificial bases in oligonucleotides has been widely used in DNA nanotechnology and biosensing technique. Using isothermal titration calorimetry, circular dichroism spectroscopy and fluorescence spectroscopy, the folding process of T-C-rich oligonucleotides (TCO) induced by Hg2+ and Ag+ with the synthetic sequence d(T6C6T6C6T6C6T6) was studied and analyzed. Although thermodynamic data predict that TCO should initially fold into a relatively stable hairpin through two possible pathways of conformational transitions whether Hg2+ or Ag+ were added at first, the mechanisms and final products between the two are entirely different from isothermal titration calorimetry outcomes. When Hg2+ were added first, the haipin was formed through T-Hg-T structure with further stabilization by C-Ag-C after Ag+ addition. However, it is proposed that an unusual metal-base pair for Ag+ binding is generated instead classical C-Ag-C when Ag+ was injected first. Moreover, further confirmation of this unconventional metal-base pair T-Ag-C was verified by circular dichroism and fluorescence spectroscopy.

Wei Deng, Bin Zheng, Wei Ding, Hong Zhu, Hao-jun Liang. Metal-Triggered DNA Folding by Different Mechanisms[J]. Chinese Journal of Chemical Physics , 2015, 28(5): 630-638. doi: 10.1063/1674-0068/28/cjcp1503051
Citation: Wei Deng, Bin Zheng, Wei Ding, Hong Zhu, Hao-jun Liang. Metal-Triggered DNA Folding by Different Mechanisms[J]. Chinese Journal of Chemical Physics , 2015, 28(5): 630-638. doi: 10.1063/1674-0068/28/cjcp1503051

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