Zheng-qiu Yuan, Jin-xing Long, Tie-jun Wang, Yu-qin Li, Qi Zhang, Long-long Ma. Cascade Enzymatic Hydrolysis Coupling with Ultra ne Grinding Pretreatment for Sugarcane Bagasse Sacchari cation[J]. Chinese Journal of Chemical Physics , 2015, 28(3): 355-360. doi: 10.1063/1674-0068/28/cjcp1502014
Citation: Zheng-qiu Yuan, Jin-xing Long, Tie-jun Wang, Yu-qin Li, Qi Zhang, Long-long Ma. Cascade Enzymatic Hydrolysis Coupling with Ultra ne Grinding Pretreatment for Sugarcane Bagasse Sacchari cation[J]. Chinese Journal of Chemical Physics , 2015, 28(3): 355-360. doi: 10.1063/1674-0068/28/cjcp1502014

Cascade Enzymatic Hydrolysis Coupling with Ultra ne Grinding Pretreatment for Sugarcane Bagasse Sacchari cation

doi: 10.1063/1674-0068/28/cjcp1502014
  • Received Date: 2015-02-03
  • The biorefinery process for sugarcane bagasse saccharification generally requires significant accessibility of cellulose. We reported a novel method of cascade cellulase enzymatic hydrolysis coupling with ultrafine grinding pretreatment for sugarcane bagasse saccharification. Three enzymatic hydrolysis modes including single cellulase enzymatic hydrolysis, mixed cellulase enzymatic hydrolysis, and cascade cellulase enzymatic hydrolysis were compared. The changes on the functional group and surface morphology of bagasse during cascade cellulase enzymatic hydrolysis were also examined by FT-IR and SEM respectively. The results showed that cascade enzymatic hydrolysis was the most efficient way to enhance the sugarcane bagasse sacchari cation. More than 65% of reducing sugar yield with 90.1% of glucose selectivity was achieved at 50 oC, pH=4.8 for 72 h (1200 r/min) with cellulase I of 7.5 FPU/g substrate and cellulase II of 5 FPU/g substrate.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Cascade Enzymatic Hydrolysis Coupling with Ultra ne Grinding Pretreatment for Sugarcane Bagasse Sacchari cation

doi: 10.1063/1674-0068/28/cjcp1502014

Abstract: The biorefinery process for sugarcane bagasse saccharification generally requires significant accessibility of cellulose. We reported a novel method of cascade cellulase enzymatic hydrolysis coupling with ultrafine grinding pretreatment for sugarcane bagasse saccharification. Three enzymatic hydrolysis modes including single cellulase enzymatic hydrolysis, mixed cellulase enzymatic hydrolysis, and cascade cellulase enzymatic hydrolysis were compared. The changes on the functional group and surface morphology of bagasse during cascade cellulase enzymatic hydrolysis were also examined by FT-IR and SEM respectively. The results showed that cascade enzymatic hydrolysis was the most efficient way to enhance the sugarcane bagasse sacchari cation. More than 65% of reducing sugar yield with 90.1% of glucose selectivity was achieved at 50 oC, pH=4.8 for 72 h (1200 r/min) with cellulase I of 7.5 FPU/g substrate and cellulase II of 5 FPU/g substrate.

Zheng-qiu Yuan, Jin-xing Long, Tie-jun Wang, Yu-qin Li, Qi Zhang, Long-long Ma. Cascade Enzymatic Hydrolysis Coupling with Ultra ne Grinding Pretreatment for Sugarcane Bagasse Sacchari cation[J]. Chinese Journal of Chemical Physics , 2015, 28(3): 355-360. doi: 10.1063/1674-0068/28/cjcp1502014
Citation: Zheng-qiu Yuan, Jin-xing Long, Tie-jun Wang, Yu-qin Li, Qi Zhang, Long-long Ma. Cascade Enzymatic Hydrolysis Coupling with Ultra ne Grinding Pretreatment for Sugarcane Bagasse Sacchari cation[J]. Chinese Journal of Chemical Physics , 2015, 28(3): 355-360. doi: 10.1063/1674-0068/28/cjcp1502014

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