An-sha Zhao, Zhao Wang, Xiao-hua Zhu, Manfred F. Maitz, Nan Huang. Real-Time Characterization of Fibrinogen Interaction with Modified Titanium Dioxide Film by Quartz Crystal Microbalance with Dissipation[J]. Chinese Journal of Chemical Physics , 2014, 27(3): 355-360. doi: 10.1063/1674-0068/27/03/355-360
Citation: An-sha Zhao, Zhao Wang, Xiao-hua Zhu, Manfred F. Maitz, Nan Huang. Real-Time Characterization of Fibrinogen Interaction with Modified Titanium Dioxide Film by Quartz Crystal Microbalance with Dissipation[J]. Chinese Journal of Chemical Physics , 2014, 27(3): 355-360. doi: 10.1063/1674-0068/27/03/355-360

Real-Time Characterization of Fibrinogen Interaction with Modified Titanium Dioxide Film by Quartz Crystal Microbalance with Dissipation

doi: 10.1063/1674-0068/27/03/355-360
Funds:  This work was supported by the National Natural Sci-ence Foundation of China (No.9732011CB606200 and No.81330031) and Fundamental Research Funds for the Central Universities (No.SWJTU11CX054). The au-thors gratefully acknowledge assistance of Mr. Hai-bei Liu at Qsense company for consulting.
  • Received Date: 2013-12-18
  • The adsorption of fibrinogen can be used as a quick indicator of surface haemocompatibility because of its prominent role in coagulation and platelet adhesion. In this work the molec-ular interaction between flbrinogen and a modified titanium oxide surface/platelet has been studied by quartz crystal microbalance with dissipation (QCM-D) in situ. In order to further characterize the conformation of adsorbed flbrinogen, αC and γ-chain antibody were used to check the orientation and denaturation of flbrinogen on solid surface. QCM-D investiga-tions revealed the flbrinogen have the trend to adsorb on hydrophilic surface in a side-on orientation by positively charged αC domains, which would reduce the exposure of platelet bonding site on γ chain and enable less platelet adhesion and be activated. These obser-vations suggest that certain conformations of adsorbed flbrinogen are less platelet adhesive than others, which opens a possibility for creating a non-platelet adhesive substrates.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(1308) PDF downloads(1465) Cited by()

Proportional views
Related

Real-Time Characterization of Fibrinogen Interaction with Modified Titanium Dioxide Film by Quartz Crystal Microbalance with Dissipation

doi: 10.1063/1674-0068/27/03/355-360
Funds:  This work was supported by the National Natural Sci-ence Foundation of China (No.9732011CB606200 and No.81330031) and Fundamental Research Funds for the Central Universities (No.SWJTU11CX054). The au-thors gratefully acknowledge assistance of Mr. Hai-bei Liu at Qsense company for consulting.

Abstract: The adsorption of fibrinogen can be used as a quick indicator of surface haemocompatibility because of its prominent role in coagulation and platelet adhesion. In this work the molec-ular interaction between flbrinogen and a modified titanium oxide surface/platelet has been studied by quartz crystal microbalance with dissipation (QCM-D) in situ. In order to further characterize the conformation of adsorbed flbrinogen, αC and γ-chain antibody were used to check the orientation and denaturation of flbrinogen on solid surface. QCM-D investiga-tions revealed the flbrinogen have the trend to adsorb on hydrophilic surface in a side-on orientation by positively charged αC domains, which would reduce the exposure of platelet bonding site on γ chain and enable less platelet adhesion and be activated. These obser-vations suggest that certain conformations of adsorbed flbrinogen are less platelet adhesive than others, which opens a possibility for creating a non-platelet adhesive substrates.

An-sha Zhao, Zhao Wang, Xiao-hua Zhu, Manfred F. Maitz, Nan Huang. Real-Time Characterization of Fibrinogen Interaction with Modified Titanium Dioxide Film by Quartz Crystal Microbalance with Dissipation[J]. Chinese Journal of Chemical Physics , 2014, 27(3): 355-360. doi: 10.1063/1674-0068/27/03/355-360
Citation: An-sha Zhao, Zhao Wang, Xiao-hua Zhu, Manfred F. Maitz, Nan Huang. Real-Time Characterization of Fibrinogen Interaction with Modified Titanium Dioxide Film by Quartz Crystal Microbalance with Dissipation[J]. Chinese Journal of Chemical Physics , 2014, 27(3): 355-360. doi: 10.1063/1674-0068/27/03/355-360

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return