Yu-ying Liu, Peng-ye Wang, Shuo-xing Dou, Hong-feng Lv. Interaction Study between DNA and Histone Proteins on Single-molecule Level using Atomic Force Microscopy[J]. Chinese Journal of Chemical Physics , 2014, 27(1): 115-120. doi: 10.1063/1674-0068/27/01/115-120
Citation: Yu-ying Liu, Peng-ye Wang, Shuo-xing Dou, Hong-feng Lv. Interaction Study between DNA and Histone Proteins on Single-molecule Level using Atomic Force Microscopy[J]. Chinese Journal of Chemical Physics , 2014, 27(1): 115-120. doi: 10.1063/1674-0068/27/01/115-120

Interaction Study between DNA and Histone Proteins on Single-molecule Level using Atomic Force Microscopy

doi: 10.1063/1674-0068/27/01/115-120
Funds:  This work was supported by the National Natural Sci-ence Foundation of China (No.11274374), the National Basic Research Program of China (No.2009CB930704), and the Basic Scientific Research Foundation of China Agricultural University (No.2012QJ026).
  • Received Date: 2013-07-30
  • DNA and histone protein are important in the formation of nucleosomal arrays, which are the first packaging level of DNA into a more compact chromatin structure. To characterize the interactions of DNA and histone proteins, we reconstitute nucleosomes using lambda DNA and whole histone proteins by dialysis and perform direct atomic force microscopy (AFM) imaging. Compared with non-specific DNA and histone binding, nucleosomes are formed within the assembled “beads-on-a-string” nucleosomal array by dialysis. These observations facilitate the establishment of the molecular mechanisms of nucleosome and demonstrate the capability of AFM for protein-DNA interaction analysis.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Interaction Study between DNA and Histone Proteins on Single-molecule Level using Atomic Force Microscopy

doi: 10.1063/1674-0068/27/01/115-120
Funds:  This work was supported by the National Natural Sci-ence Foundation of China (No.11274374), the National Basic Research Program of China (No.2009CB930704), and the Basic Scientific Research Foundation of China Agricultural University (No.2012QJ026).

Abstract: DNA and histone protein are important in the formation of nucleosomal arrays, which are the first packaging level of DNA into a more compact chromatin structure. To characterize the interactions of DNA and histone proteins, we reconstitute nucleosomes using lambda DNA and whole histone proteins by dialysis and perform direct atomic force microscopy (AFM) imaging. Compared with non-specific DNA and histone binding, nucleosomes are formed within the assembled “beads-on-a-string” nucleosomal array by dialysis. These observations facilitate the establishment of the molecular mechanisms of nucleosome and demonstrate the capability of AFM for protein-DNA interaction analysis.

Yu-ying Liu, Peng-ye Wang, Shuo-xing Dou, Hong-feng Lv. Interaction Study between DNA and Histone Proteins on Single-molecule Level using Atomic Force Microscopy[J]. Chinese Journal of Chemical Physics , 2014, 27(1): 115-120. doi: 10.1063/1674-0068/27/01/115-120
Citation: Yu-ying Liu, Peng-ye Wang, Shuo-xing Dou, Hong-feng Lv. Interaction Study between DNA and Histone Proteins on Single-molecule Level using Atomic Force Microscopy[J]. Chinese Journal of Chemical Physics , 2014, 27(1): 115-120. doi: 10.1063/1674-0068/27/01/115-120

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