Bo Wu, Yan-qiu Chu, Zhao-yun Dai, Chuan-fan Ding¤. Interaction Between Cytochrome c and the Hapten 2,4-Dinitro-fluorobenzene by Electrospray Ionization Mass Spectrometry[J]. Chinese Journal of Chemical Physics , 2008, 21(3): 217-220. doi: 10.1088/1674-0068/21/03/217-220
Citation: Bo Wu, Yan-qiu Chu, Zhao-yun Dai, Chuan-fan Ding¤. Interaction Between Cytochrome c and the Hapten 2,4-Dinitro-fluorobenzene by Electrospray Ionization Mass Spectrometry[J]. Chinese Journal of Chemical Physics , 2008, 21(3): 217-220. doi: 10.1088/1674-0068/21/03/217-220

Interaction Between Cytochrome c and the Hapten 2,4-Dinitro-fluorobenzene by Electrospray Ionization Mass Spectrometry

doi: 10.1088/1674-0068/21/03/217-220
Funds:  This work was supported by the National Natural Science Foundation of China (No.20473020).
  • Received Date: 2007-12-12
  • Rev Recd Date: 2008-01-30
  • Allergic contact dermatitis is a delayed hypersensitivity reaction, which results from skin exposure to low molecular weight chemicals such as haptens. To clarify the pathogenic mechanism, electrospray ionization mass spectrometry (ESI-MS) and hydrogen/deuterium (H/D) exchange, as well as UV spectroscopy, were applied to determine the interaction between the model protein cytochrome c (cyt c) and the hapten 2,4-dinitro-fluorobenzene (DNFB). The ESI-MS results demonstrate that the conformation of cyt c can change from native folded state into partially unfolded state with the increase of DNFB. The equilibrium state H/D exchange followed by ESI-MS further confirms the above results. UV spectroscopy indicates that the strongfield coordination between iron of heme (prosthetic group) and His18 or Met80 of cyt c is not obviously affected by the hapten.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Interaction Between Cytochrome c and the Hapten 2,4-Dinitro-fluorobenzene by Electrospray Ionization Mass Spectrometry

doi: 10.1088/1674-0068/21/03/217-220
Funds:  This work was supported by the National Natural Science Foundation of China (No.20473020).

Abstract: Allergic contact dermatitis is a delayed hypersensitivity reaction, which results from skin exposure to low molecular weight chemicals such as haptens. To clarify the pathogenic mechanism, electrospray ionization mass spectrometry (ESI-MS) and hydrogen/deuterium (H/D) exchange, as well as UV spectroscopy, were applied to determine the interaction between the model protein cytochrome c (cyt c) and the hapten 2,4-dinitro-fluorobenzene (DNFB). The ESI-MS results demonstrate that the conformation of cyt c can change from native folded state into partially unfolded state with the increase of DNFB. The equilibrium state H/D exchange followed by ESI-MS further confirms the above results. UV spectroscopy indicates that the strongfield coordination between iron of heme (prosthetic group) and His18 or Met80 of cyt c is not obviously affected by the hapten.

Bo Wu, Yan-qiu Chu, Zhao-yun Dai, Chuan-fan Ding¤. Interaction Between Cytochrome c and the Hapten 2,4-Dinitro-fluorobenzene by Electrospray Ionization Mass Spectrometry[J]. Chinese Journal of Chemical Physics , 2008, 21(3): 217-220. doi: 10.1088/1674-0068/21/03/217-220
Citation: Bo Wu, Yan-qiu Chu, Zhao-yun Dai, Chuan-fan Ding¤. Interaction Between Cytochrome c and the Hapten 2,4-Dinitro-fluorobenzene by Electrospray Ionization Mass Spectrometry[J]. Chinese Journal of Chemical Physics , 2008, 21(3): 217-220. doi: 10.1088/1674-0068/21/03/217-220

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