Zhang-yi Liang, Ning Xu, Ying-hua Guan, You-yi Zhang, Xin-sheng Zhao. Mode of Myosin Transportation in Living Cells Studied by Single Particle Tracking[J]. Chinese Journal of Chemical Physics , 2007, 20(4): 445-448. doi: 10.1088/1674-0068/20/04/445-448
Citation: Zhang-yi Liang, Ning Xu, Ying-hua Guan, You-yi Zhang, Xin-sheng Zhao. Mode of Myosin Transportation in Living Cells Studied by Single Particle Tracking[J]. Chinese Journal of Chemical Physics , 2007, 20(4): 445-448. doi: 10.1088/1674-0068/20/04/445-448

Mode of Myosin Transportation in Living Cells Studied by Single Particle Tracking

doi: 10.1088/1674-0068/20/04/445-448
Funds:  This work was supported by the National Natu-ral Science Foundation of China (No.20333010 andNo.30490172) and the National Key Basic Re-search Special Funds project (No.2006CB910300 andNo.2003CB716900).
  • Received Date: 2007-05-08
  • Rev Recd Date: 2007-06-19
  • The transport of internalized ?1A-adrenergic receptor (?1A-AR) by myosin protein in live cells was studied. The technique of single particle tracking by °uorescence imaging with high temporal and spatial resolution was used. The endosomes of ?1A-AR were transported along actin ˉlaments in a step-by-step mode. The average step-size in di?erent time resolutions is consistent with the step-size of myosin assay in vitro. With the simulation of the stepwise traces in di?erent time resolutions, we found that the kinetic process of each step is in coherence with the single myosin assay in vitro.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Mode of Myosin Transportation in Living Cells Studied by Single Particle Tracking

doi: 10.1088/1674-0068/20/04/445-448
Funds:  This work was supported by the National Natu-ral Science Foundation of China (No.20333010 andNo.30490172) and the National Key Basic Re-search Special Funds project (No.2006CB910300 andNo.2003CB716900).

Abstract: The transport of internalized ?1A-adrenergic receptor (?1A-AR) by myosin protein in live cells was studied. The technique of single particle tracking by °uorescence imaging with high temporal and spatial resolution was used. The endosomes of ?1A-AR were transported along actin ˉlaments in a step-by-step mode. The average step-size in di?erent time resolutions is consistent with the step-size of myosin assay in vitro. With the simulation of the stepwise traces in di?erent time resolutions, we found that the kinetic process of each step is in coherence with the single myosin assay in vitro.

Zhang-yi Liang, Ning Xu, Ying-hua Guan, You-yi Zhang, Xin-sheng Zhao. Mode of Myosin Transportation in Living Cells Studied by Single Particle Tracking[J]. Chinese Journal of Chemical Physics , 2007, 20(4): 445-448. doi: 10.1088/1674-0068/20/04/445-448
Citation: Zhang-yi Liang, Ning Xu, Ying-hua Guan, You-yi Zhang, Xin-sheng Zhao. Mode of Myosin Transportation in Living Cells Studied by Single Particle Tracking[J]. Chinese Journal of Chemical Physics , 2007, 20(4): 445-448. doi: 10.1088/1674-0068/20/04/445-448

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