Abstract: Highly efficient synthesis of clean biofuels using the bio-syngas obtained from biomass gasi-fication was performed over Fe_1.5Cu₁Zn₁Al₁K_0.117 catalyst. The maximum biofuel yield from the bio-syngas reaches about 1.59 kg biofuels/(kgcatal·h) with a contribution of 0.57 kg alcohols/(kgcatal·h) and 1.02 kg liquid hydrocarbons/(kgcatal·h). The alcohol products in the resulting biofuels were dominated by the C2⁺ alcohols (mainly C2-C6 alcohols) with a content of 73.55%-89.98%. The selectivity of the liquid hydrocarbons (C5⁺) in the hy-drocarbon products ranges from 60.37% to 70.94%. The synthesis biofuels also possess a higher heat value of 40.53-41.49 MJ/kg. The effects of the synthesis conditions, including temperature, pressure, and gas hourly space velocity, on the biofuel synthesis were investi-gated in detail. The catalyst features were characterized by inductively coupled plasma and atomic emission spectroscopy, X-ray diffraction, temperature programmed reduction, and the N₂ adsorption-desorption isotherms measurements. The present biofuel synthesis with a higher biofuel yield and a higher selectivity of liquid hydrocarbons and C2⁺ alcohols may be a potentially useful route to produce clean biofuels and chemicals from biomass.