The Effect of Deoxyribosyl Raman Lines for the DNA Conformational Determination

In laser Raman spectrum of calf thymus DNA aqueous solution, the characteristical lines 671 and 682 cm^-1 of deoxyguanosine sugsr ring packers, which expressed C3’ endo/anti and C2’ endo/anti conformation respectively, have been obtained Simultaneously, thus indicates that two type spatial conformation（A-and B- DNA） exsist in the DNA aqueous solution. The calculated relstive content at 671 cm^-1 of marker C3’-endo/anti dG is 39. 4%, be clack to the estimated 35% A-DNA content by the own modified Brown’s method. The same conclusions have been qualitative confirmed with the other Raman frequencies diagnostic of both deoxyribeyl conformers and deoxyribosyl phosphoate backbone vibretions.This result will be of use for thoroughly reveals the interactive mechanism between the DNA and other substance molecules.