Research of Spectra Characterization of Hydrolutic Enzymes Model Molecule

The fluorescence and electornic absorption spectra of (tris（2-benzimidazylmethyl ）amine and its Cu （Ⅱ）, Co （Ⅱ）, Zn （Ⅱ） complexes that can catalyze hydrolytic have been studied.The electronic transitions of NTB and its metal complexes were analyzed, the absorption bands in a range of 270~290nm and at about 300nm can be assigned to π→ π^* and n →π^* respectively. The quantum yields were calculated . The quantum yields were calculated . The quantum yields of the NTB-Zn was much higher than that of the NTB-Cu and NTB-Co.The hydrolytic catalyzing rate of NTB-Cu and NTB-Co were higher than that of NTB-Zn.It is shown that the hydrolytic catalyzing rate decreased with the increase of the fluorescence quantum yield. The hydrolytic catalyzing rates of Cu（Ⅱ）, Co（Ⅱ）, Zn（Ⅱ ） complexes were reduced when the pH values were raised. And the fluorescence quantum yields were raised when the pH values wer raised. It’s testified that the conclusion were right further.