A Study on the Mechanism of the Interaction between Chitosan and Zincon

In order to know the mechanism of the interaction between polysaccharides and small molecules at the molecular level, the interaction of Chitosan (CTS) and Zincon (ZCN) was studied by absorption spectra. The influence of the molar ratio of ZCN/CTS on the spectra was investigated. The critical molar ratio (2.67×103) necessary for the new absorption peak of the absorption spectra of ZCN-CTS complex and the maximum binding number (6.93×103) were obtained by spectrometry. The maximum binding number is consistent with the theoretical value. And it has proved that the theoretical model and the mechanism of the interaction between polysaccharides and biological probes are reliable. The mechanism of the color changes of ZCN and Chitosan reaction system has been discussed. We draw the conclusion that the color changes result mainly from the hydrophobic interaction between ZCN and Chitosan on the basis of the electrostatic interaction between ZCN and Chitosan.