Freeze-etching Replication Transmission Electron Microscopy In-situ Observation of the Non-aqueous Nano-dispersion System

The step and approach of specimen making for freeze-etching replication transmission electron microscopy (FERTEM) that was used for the in-situ observation of in-situ synthesizing nano-dispersion system were studied. The diameter, diameter distribution and conglomeration behavior of nano particles in foursorts of dispersion system were observed by FERTEM technique. The results indicated that the image qualityof transmission electronmicroscopy was closely related to the freeze-ecthing time, and the freeze-ecthing time should correspond to the content and diameter of nanoparticles in the dispersion system. The FERTEM technique provided a reliable means for thein-situ observation of the non-aqueous nano-dispersion system, and can collect manifold information simultaneously.