Tuning Gene Expression by Hairpin Elements near the Start Codon of mRNA in Mammalian Cells

Xue Yin; Dong-bao Yao; Hao-jun Liang

doi:10.1063/1674-0068/cjcp2207107

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Xue Yin, Dong-bao Yao, Hao-jun Liang. Tuning Gene Expression by Hairpin Elements near the Start Codon of mRNA in Mammalian Cells[J]. Chinese Journal of Chemical Physics . doi: 10.1063/1674-0068/cjcp2207107

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Xue Yin, Dong-bao Yao, Hao-jun Liang. Tuning Gene Expression by Hairpin Elements near the Start Codon of mRNA in Mammalian Cells[J]. Chinese Journal of Chemical Physics . doi: 10.1063/1674-0068/cjcp2207107

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Tuning Gene Expression by Hairpin Elements near the Start Codon of mRNA in Mammalian Cells

doi: 10.1063/1674-0068/cjcp2207107

Xue Yin^{1, 2},
Dong-bao Yao^{1
,
,},
Hao-jun Liang^{1
,
,}

1.
School of Chemistry and Materials Science, Department of Polymer Science and Engineering, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei 230026, China
2.
Department of Chemistry, City University of Hong Kong, Hong Kong 999077, China

More Information

Author Bio:
Prof. Liang passed away in February, 2022. This work is our tribute to our dear supervisor
Corresponding author: E-mail: dbyao@ustc.edu.cn; hjliang@ustc.edu.cn
Received Date: 2022-07-06
Accepted Date: 2022-09-01
Rev Recd Date: 2022-08-22

Available Online: 2022-09-03

Abstract

Abstract

The hairpin element (HpE) near the start codon in the 5′ UTR was developed to tune the mRNA translation in mammalian cells. The parameters of HpEs including thermodynamic stability, GC content, and distance between HpEs and the 5′ cap were investigated. The parameters of HpEs including thermodynamic stability, GC content, and distance between HpEs and the 5′ cap were investigated, which influenced the mRNA expression level. In addition, the start codon and the upstream open reading frame sequestered within the structures of HpEs also reduced the translation initiation. In summary, this study shows that the simple engineering HpE structure can be efficiently adopted for gene expression regulation. The predictable controllability of this simple cloning strategy can potentially achieve precise gene expression regulation in different mammalian cell types.
- Gene expression regulation,
- Hairpin element,
- Start codon,
- mRNA

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References(35)

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